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AI Summary of Peer-Reviewed Research

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HPTLC method reliably measured gentamicin sulphate in ointment

A close-up photograph of a 96-well microtiter plate containing samples with varying concentrations of red/pink liquid being held by a person wearing purple nitrile gloves, commonly used in analytical chemistry and pharmaceutical quality control testing.
Research area:ChromatographyAnalytical Methods in PharmaceuticalsAnalytical Chemistry and Chromatography

What the study found

A stability-indicating high-performance thin-layer chromatography (HPTLC) method was developed and validated for identifying and quantifying gentamicin sulphate in an ointment. The method separated gentamicin sulphate bands without interference from ointment excipients or degradation products.

What the authors say this matters
The authors conclude that the method is simple and convenient for quality control of gentamicin ointments. They note that it does not require sample pre-treatment, such as extraction from the ointment base.

What the researchers tested

The researchers developed an HPTLC method using silica gel 60 F254 plates as the stationary phase and methanol: chloroform: ammonia solution (25%) in a 1:1:1 ratio as the mobile phase. They dissolved the ointment sample in chloroform: methanol (9:1, v/v) and validated the method according to International Council for Harmonisation (ICH) guidelines.

What worked and what didn't

The method showed specificity by separating all three bands of gentamicin sulphate under photolytic, photolytic and oxidative, oxidative, acidic, and alkaline stress conditions. It also showed linearity from 50–300 ng/band with R2 ≥ 0.99, and the reported limits of detection and quantification were 7.10 ng and 21.53 ng, respectively.

What to keep in mind

The abstract does not describe broader testing beyond the ointment formulation studied. It also does not provide additional limitations beyond the method details reported here.

Key points

  • The study developed and validated an HPTLC method for gentamicin sulphate in an ointment.
  • The method separated gentamicin sulphate without interference from excipients or degradation products.
  • Validation followed ICH guidelines and showed linearity from 50–300 ng/band with R2 ≥ 0.99.
  • The reported limits were 7.10 ng for detection and 21.53 ng for quantification.
  • The authors say the method is simple and convenient and does not need sample pre-treatment.

Disclosure

Research title:
HPTLC method reliably measured gentamicin sulphate in ointment
Authors:
K. M. Yasif Kayes Sikdar, Md Khairul Islam, Edith K. Y. Tang, Tomislav Sostaric, Lee Yong Lim, Cornelia Locher
Institutions:
The University of Western Australia
Publication date:
2026-03-09
DOI:
10.3390/app16052613
OpenAlex record:
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AI provenance: This post was generated by OpenAI. The original authors did not write or review this post.

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