What the study found
The study found that an RP-HPLC (reverse-phase high-performance liquid chromatography) method could detect and quantify metoprolol and enalapril maleate in bulk and pharmaceutical products, even when degradants were present. The method was reported to give accurate and stable results and to use a sustainable approach.
Why the authors say this matters
The authors say the work matters because it offers a sustainable alternative to toxic chemicals and reduces waste while supporting analysis of a combination therapy containing metoprolol and enalapril maleate. They also state that co-administration in a single formulation is important for blood pressure control and cardiovascular risk reduction.
What the researchers tested
The researchers developed an RP-HPLC method in isocratic mode using an InertSustain C8 column. They used a 50:50 mobile phase of potassium phosphate buffer at pH 2.5 and ethanol, with a 7-minute runtime, 20 µL injection volume, 1.0 mL/min flow rate, a sample temperature of 5°C, and a column oven at 40°C.
What worked and what didn't
The method showed linearity for both metoprolol and enalapril maleate over 3–60 µg/mL, with R2 ≥ 0.999. Recovery studies were 98–102%, and the method met ICH stability criteria; enalapril maleate had a detection limit of 0.29 µg/mL and quantification limit of 0.90 µg/mL, while metoprolol had a detection limit of 0.33 µg/mL and quantification limit of 0.98 µg/mL. The abstract says the method still accurately detected and quantified both drugs despite degradants.
What to keep in mind
The available summary does not describe detailed limitations or comparisons with other methods. It also does not provide full experimental conditions for degradation testing beyond noting that degradants were present.
Key points
- The study reports an RP-HPLC method for detecting and quantifying metoprolol and enalapril maleate.
- The method was used for bulk material and pharmaceutical products.
- Linearity was reported across 3–60 µg/mL for both drugs, with R2 ≥ 0.999.
- Recovery studies were 98–102%, and the method met ICH stability criteria.
- The abstract says the method remained accurate in the presence of degradants.
Disclosure
- Research title:
- RP-HPLC accurately quantified metoprolol and enalapril maleate
- Authors:
- Ghadah M. Al-Senani, Salhah D. Al-Qahtani, Samar M. Mahgoub, Aya Shaban, Hoda A. Ahmed, Mahmoud A. Mohamed
- Institutions:
- Princess Nourah bint Abdulrahman University, Beni-Suef University, Cairo University, Taibah University, Hikma Pharmaceuticals (United Kingdom)
- Publication date:
- 2026-03-31
- OpenAlex record:
- View
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