What the study found
The study found that a reverse-phase high-performance liquid chromatography (RP-HPLC) method could be developed and validated to measure caffeine, eugenol, and zingerone together in a marketed Ashwagandha green tea product. The authors report that the method was simple, precise, specific, and robust.
Why the authors say this matters
The authors say the method can be used for standardising the three phytoconstituents in TATA TE-A-ME Ashwagandha Green Tea. They conclude that combining design of experiments (DOE) with RP-HPLC supports development of an analytical method for estimating these compounds.
What the researchers tested
The researchers developed and validated an RP-HPLC method using a C-18 column and a methanol:water mobile phase in a 35:65 v/v ratio. Detection and quantification were done at 254 nm, and robustness was addressed using design of experiments (DOE), a structured approach for testing how conditions affect a method.
What worked and what didn't
The method gave retention times of 4.76, 8.64, and 10.48 for caffeine, eugenol, and zingerone, respectively. It was validated according to ICH guidelines for linearity, precision, specificity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and robustness, and the calibration plots showed satisfactory linear relationships. The abstract does not report any failed steps or unmet validation criteria.
What to keep in mind
The abstract focuses on one marketed formulation and does not describe broader testing beyond that product. It also does not provide detailed numerical validation data in the available summary.
Key points
- An RP-HPLC method was developed to measure caffeine, eugenol, and zingerone in Ashwagandha green tea.
- The method used a C-18 column, a methanol:water mobile phase (35:65 v/v), and detection at 254 nm.
- Retention times were reported as 4.76 minutes for caffeine, 8.64 minutes for eugenol, and 10.48 minutes for zingerone.
- Validation followed ICH guidelines and included linearity, precision, specificity, LOD, LOQ, accuracy, and robustness.
- The abstract states the method was simple, precise, specific, and robust.
Disclosure
- Research title:
- Validated HPLC method estimates three tea phytoconstituents
- Authors:
- Vrutika Patel, Tanvi Dodiya, Disha Prajapati
- Institutions:
- Parul University
- Publication date:
- 2026-02-26
- OpenAlex record:
- View
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